Coding

Part:BBa_K3867001:Design

Designed by: Lei Huang   Group: iGEM21_ICJFLS   (2021-10-13)


ADG1 （ADP glucose pyrophosphorylase 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use two restriction enzymes EcoRI and PstI to insert the ADG1 gene into the vector, so these two enzyme sits are avoided.


Source

It comes from the rDNA sequence of Arabidopsis thaliana.

References